Sphingosine Kinase 1 + ERK2 Lysate
€179.00
In stock
SKU
ECM-SL9161
Background:
Sphingosine Kinase (SK) catalyzes the phosphorylation of the lipid sphingosine, creating S1P. S1P subsequently signals through cell surface G protein-coupled receptors, as well as intracellularly, to modulate cell proliferation, survival, motility and differentiation. Regulation of SK1 may occur through ERK mediated phosphorylation of Ser-225. This lysate was generated from human recombinant sphingosine kinase 1 that had been phosphorylated by ERK-2 kinase in an in vitro kinase reaction. The lysate can be loaded on mini-gels at 10-20 ml/lane and used as a positive control in western blots probed with anti-sphingosine kinase 1 (SP1621) or anti-sphingosine kinase 1 (Ser-225), phospho-specific (SP1641).
Buffer/Storage:
SK1 Lysate is supplied at a concentration of 2 ng/µl in electrophoresis sample buffer (62.5 mM Tris pH 6.8, 2% SDS, 5% glycerol, 0.003% bromophenol blue, 0.9% β-mercaptoethanol). Store at –20°C. Do not boil or dilute. Stable for 1 year.
Sphingosine Kinase (SK) catalyzes the phosphorylation of the lipid sphingosine, creating S1P. S1P subsequently signals through cell surface G protein-coupled receptors, as well as intracellularly, to modulate cell proliferation, survival, motility and differentiation. Regulation of SK1 may occur through ERK mediated phosphorylation of Ser-225. This lysate was generated from human recombinant sphingosine kinase 1 that had been phosphorylated by ERK-2 kinase in an in vitro kinase reaction. The lysate can be loaded on mini-gels at 10-20 ml/lane and used as a positive control in western blots probed with anti-sphingosine kinase 1 (SP1621) or anti-sphingosine kinase 1 (Ser-225), phospho-specific (SP1641).
Buffer/Storage:
SK1 Lysate is supplied at a concentration of 2 ng/µl in electrophoresis sample buffer (62.5 mM Tris pH 6.8, 2% SDS, 5% glycerol, 0.003% bromophenol blue, 0.9% β-mercaptoethanol). Store at –20°C. Do not boil or dilute. Stable for 1 year.
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