STAT1 (Phospho-S727) polyclonal, anti-human, mouse, rat

Background:
Membrane receptor signaling by various ligands, including interferons and growth hormones such as EGF, induces activation of Jak kinases which then leads to tyrosine phosphorylation of the various Stat transcription factors. Stat1 and Stat2 are induced by IFN-a and form a heterodimer which is part of the ISGF3 transcription factor complex. Although early reports indicate Stat3 activation by EGF and IL-6, it has been shown that Stat3β appears to be activated by both while Stat3α is activated by EGF, but not by IL-6. Highest expression of Stat4 is seen in testis and myeloid cells. IL-12 has been identified as an activator of Stat4. Stat5 has been shown to be activated by prolactin and by IL-3. Stat6 is involved in IL-4 activated signaling pathways.

Alternative Name:
STAT1, Signal transducer and activator of transcription 1 91kD, CANDF7, DKFZp686B04100, IMD31A, IMD31B, IMD31C, ISGF 3, ISGF-3, OTTHUMP00000163552, OTTHUMP00000165046, OTTHUMP00000165047, OTTHUMP00000205845, Signal transducer and activator of transcription 1 91kDa, Signal transducer and activator of transcription 1, Signal transducer and activator of transcription 1, 91kD, Signal transducer and activator of transcription 1-alpha/beta, STAT 1, Stat1, STAT1, STAT91, Transcription factor ISGF 3 components p91 p84, Transcription factor ISGF-3 components p91/p84,

Application Dilution:
WB:1:1,000
IHC:1:50-1:200

Specificity: STAT1 (Phospho-S727) polyclonal antibody detects endogenous levels of STAT1 protein only when phosphorylated at S727.

Immunogen:
Synthetic phospho-peptide corresponding to residues surrounding Ser727 of human STAT1.

MW: 87 kDa

Swiss-Prot: P42224(Human) P42225(Mouse) Unigene:33229(Rat)

Purification & Purity:
ProA affinity purified

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

For research use only, not for use in diagnostic procedure.