Stat5 (unphosphorylated Tyr-694) Blocking Peptide
€155.00
In stock
SKU
ECM-SX2205
Background:
The stat proteins function both as cytoplasmic signal transducers and as activators of transcription. Stat5 is activated in response to a wide variety of ligands including IL-2, GM-CSF, growth hormone and prolactin. Phosphorylation at Tyr-694 is required for Stat5A activation. Stat5 has been found to be constitutively active in some leukemic cell types. Phosphorylated Stat5 is found in some endothelial cells treated with IL-3, which suggests its involvement in angiogenesis and cell motility. Both Stat5A (Tyr-694) and Stat5B (Tyr-699) are independently regulated and activated in various cell types. For instance, both isoforms are activated in response to IFNα in B cells, but only Stat5A is phosphorylated in response to IFNα in HeLa cells.
Sequence: Unphosphorylated Stat5 (Tyr-694) synthetic peptide corresponds to amino acid residues around tyrosine 694 of human Stat5A. This peptide sequence is identical to tyrosine 699 in human Stat5B, and has high homology to the conserved tyrosine site in rat and mouse Stat5A/B.
Specificity: This peptide is an unphosphorylated control peptide for Stat5 (Tyr-694) phospho-specific antibody (SM1481). The peptide can be used along with phosphorylated Stat5 (Tyr-694) peptide (SX1485) in ELISA, Western blot and immunocytochemistry antibody blocking experiments.
Buffer/Storage:
Blocking Peptide is supplied in 50µl phosphate-buffered saline and 0.05% sodium azide. Store at –20°C. Stable for 1 year.
The stat proteins function both as cytoplasmic signal transducers and as activators of transcription. Stat5 is activated in response to a wide variety of ligands including IL-2, GM-CSF, growth hormone and prolactin. Phosphorylation at Tyr-694 is required for Stat5A activation. Stat5 has been found to be constitutively active in some leukemic cell types. Phosphorylated Stat5 is found in some endothelial cells treated with IL-3, which suggests its involvement in angiogenesis and cell motility. Both Stat5A (Tyr-694) and Stat5B (Tyr-699) are independently regulated and activated in various cell types. For instance, both isoforms are activated in response to IFNα in B cells, but only Stat5A is phosphorylated in response to IFNα in HeLa cells.
Sequence: Unphosphorylated Stat5 (Tyr-694) synthetic peptide corresponds to amino acid residues around tyrosine 694 of human Stat5A. This peptide sequence is identical to tyrosine 699 in human Stat5B, and has high homology to the conserved tyrosine site in rat and mouse Stat5A/B.
Specificity: This peptide is an unphosphorylated control peptide for Stat5 (Tyr-694) phospho-specific antibody (SM1481). The peptide can be used along with phosphorylated Stat5 (Tyr-694) peptide (SX1485) in ELISA, Western blot and immunocytochemistry antibody blocking experiments.
Buffer/Storage:
Blocking Peptide is supplied in 50µl phosphate-buffered saline and 0.05% sodium azide. Store at –20°C. Stable for 1 year.
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