Sucrose Fluorometric Assay Kit

Detection Principle:
Sucrose can be hydrolyzed by sucrase to produce glucose under acidic conditions, which is catalyzed by glucose oxidase to produce hydrogen peroxide. In the presence of HRP (horse radish peroxidase), hydrogen peroxide reacts with the fluorescent probe to form red fluorescent substance. The sucrose content can be calculated by measuring the fluorescence intensity at the excitation wavelength of 535 nm and the emission wavelength of 590 nm.
Instrument: Fluorescence microplate reader
Assay Time: 70 min

Tested Sample Types: Plant tissue

Research Area's: Others

Shelf Life: 6 Months

Storage:
-20℃