Talin Phospho-Regulation Antibody Sampler Kit

Background:
Talin is an important cytoskeletal component of integrin adhesion sites. Calpins cleave talin precursor (240 kDa) into an amino-terminal globular head domain of 47 kDa and a carboxyl-terminal 190 kDa rod domain. The talin head domain contains a FERM domain, that binds integrins, PIP kinase (Type I), and FAK. The rod domain has several vinculin-binding sites, a second integrin-binding site, and two actin-binding sites. These talin protein-protein interactions are critical for integrin activation, focal adhesion formation and cell migration. Talin regulation may occur through phosphorylation and regulated degradation. The talin head domain binds Smurf1, an E3 ubiquitin ligase, and this interaction leads to talin head ubiquitylation and degradation. Cdk5 can phosphorylate Ser-425 in the head domain, and this inhibits both binding to Smurf1 and subsequent degradation. The S425A talin mutant resists Cdk5 phosphorylation, increases susceptibility to Smurf1-mediated ubiquitylation, and inhibits cell migration. Thus, talin head phosphorylation may be important for regulating adhesion stability and cell migration.

Background References
Ratnikov, B. et al. (2005) J. Cell Sci. 118:4921.
Moser, M. et al. (2009) Science. 324(5929):895.
Huang, C. et al. (2009) Nat Cell Biol. 11(5):624.

Buffer/Storage:
Rabbit polyclonal and mouse monoclonal antibodies are supplied in phosphate-buffered saline (PBS), 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. The secondary reagents are supplied in the same buffer without azide, and the peptide is supplied in PBS and 0.05% sodium azide. Store all at –20°C. Stable for 1 year.

Product References:
TP4171 Jin, J.K. et al. (2015) Oncogene. 34(14): 1811. WB: metastatic PCa cells, PC3-MM2, shRNA
TX4175 Jin, J.K. et al. (2015) Oncogene. 34(14): 1811. WB: metastatic PCa cells, PC3-MM2, shRNA
RS3251 Kawasaki, H. et al. (2013) World J Gastroenter. 19(17):2629. WB, ICC: mouse intestinal myofibroblasts
MS3001 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot
RS3251 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot