TFIIB (clone ), anti-human
€388.00
In stock
SKU
MB0045
Background:
Such factors can be divided into two major functional classes: the basal factors that are required for transcription of all Pol II genes, including TFIIA, TFIIB, TFIID, TFIIE, TFIIF and TFIIH; and sequence-specific factors that regulate gene expression. The basal transcription factors and Pol II form a specific multiprotein complex near the transcription start site by interacting with core promotor elements such as the TATA box generally located 25-30 base pairs upstream of the transcription start site. Template commitment is established by the initial binding of TFIID to the “TATA” element of the promotor, a step which may be facilitated by TFIIA. TFIIB then acts as the bridge between TFIID and RNA polymerase II.
Alternative Name:
TF2B, TFIIB, S300-II, Transcription initiation factor IIB
Application Dilution: WB: 1:1000, ICC: 1:50~200, IHC: 1:500
Specificity: This antibody detects endogenous levels of TFIIB and does not cross-react with related proteins
Immunogen:
Recombinant full length Human TFIIB.
MW: Predicted band size: 35KDa, Observed band size: 37KDa
Swis Prot.: Q00403
Purification & Purity:
The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Format:
Mouse IgG2b. Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50%,glycerol
Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
For research use only, not for use in diagnostic procedure.
Such factors can be divided into two major functional classes: the basal factors that are required for transcription of all Pol II genes, including TFIIA, TFIIB, TFIID, TFIIE, TFIIF and TFIIH; and sequence-specific factors that regulate gene expression. The basal transcription factors and Pol II form a specific multiprotein complex near the transcription start site by interacting with core promotor elements such as the TATA box generally located 25-30 base pairs upstream of the transcription start site. Template commitment is established by the initial binding of TFIID to the “TATA” element of the promotor, a step which may be facilitated by TFIIA. TFIIB then acts as the bridge between TFIID and RNA polymerase II.
Alternative Name:
TF2B, TFIIB, S300-II, Transcription initiation factor IIB
Application Dilution: WB: 1:1000, ICC: 1:50~200, IHC: 1:500
Specificity: This antibody detects endogenous levels of TFIIB and does not cross-react with related proteins
Immunogen:
Recombinant full length Human TFIIB.
MW: Predicted band size: 35KDa, Observed band size: 37KDa
Swis Prot.: Q00403
Purification & Purity:
The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Format:
Mouse IgG2b. Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50%,glycerol
Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
For research use only, not for use in diagnostic procedure.
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