TFIIE-β polyclonal, anti-human, mouse, rat
€305.00
In stock
SKU
BS5964
Background:
In eukaryotic systems, initiation of transcription from protein-coding genes is a complex process requiring RNA polymerase II and broad families of auxiliary transcription factors. Such factors can be divided into two major functional classes: the basal factors that are required for transcription of all Pol II genes, including TFIIA, TFIIB, TFIID, TFIIE, TFIIF and TFIIH; and sequencespecific factors that regulate gene expression. The basal transcription factors and Pol II form a specific multiprotein complex near the transcriptional start site by interacting with core promotor elements such as the TATA box generally located 25-30 base pairs upstream of the transcription start site. Human TFIIE consists of two subunits of 56 kDa and 34 kDa molecular weight, respectively. The structure of TFIIE appears to be a heterotetramer(α2 β2) both subunits being required for optimal basal-level transcription .
Alternative Name:
Transcription initiation factor IIE subunit beta, TFIIE-beta, General transcription factor IIE subunit 2, GTF2E2, TF2E2, TFIIE β, TFIIEβ,
Application Dilution: WB: 1:500~1:1000, IHC: 1:50~1:200
Specificity: TFIIE-β polyclonal antibody detects endogenous levels of TFIIE-β protein.
Immunogen:
Synthetic peptide, corresponding to amino acids 193-238 of Human TFIIE-β.
MW: ~ 33 kDa
Swis Prot.: P29084
Purification & Purity:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Format:
1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
For research use only, not for use in diagnostic procedure.
In eukaryotic systems, initiation of transcription from protein-coding genes is a complex process requiring RNA polymerase II and broad families of auxiliary transcription factors. Such factors can be divided into two major functional classes: the basal factors that are required for transcription of all Pol II genes, including TFIIA, TFIIB, TFIID, TFIIE, TFIIF and TFIIH; and sequencespecific factors that regulate gene expression. The basal transcription factors and Pol II form a specific multiprotein complex near the transcriptional start site by interacting with core promotor elements such as the TATA box generally located 25-30 base pairs upstream of the transcription start site. Human TFIIE consists of two subunits of 56 kDa and 34 kDa molecular weight, respectively. The structure of TFIIE appears to be a heterotetramer(α2 β2) both subunits being required for optimal basal-level transcription .
Alternative Name:
Transcription initiation factor IIE subunit beta, TFIIE-beta, General transcription factor IIE subunit 2, GTF2E2, TF2E2, TFIIE β, TFIIEβ,
Application Dilution: WB: 1:500~1:1000, IHC: 1:50~1:200
Specificity: TFIIE-β polyclonal antibody detects endogenous levels of TFIIE-β protein.
Immunogen:
Synthetic peptide, corresponding to amino acids 193-238 of Human TFIIE-β.
MW: ~ 33 kDa
Swis Prot.: P29084
Purification & Purity:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Format:
1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
For research use only, not for use in diagnostic procedure.
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