TGF-alpha (clone 1E8-G6), anti-human, rabbit, zebrafish

Source:
A BALB/c mouse was immunized with synthetic TGF-alpha 34-43 decapeptide linked carrier. Fusion partner Sp2/0-Ag14.

Specifications:
1E8-G6 reacts with TGF-alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. 1E8-G6 is completely blocked by the peptide used for immunization. TGF-alpha (aa50) is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype. In sections both cytoplasma and cell membranes are stained.

Species reactivity:
Positive: human, rabbit, zebrafish.

Applications:
Detection of TGF-alpha.
ELISA: +
Flow cytometry: +
Frozen sections: +
Paraffin sections: citrate
Western blotting: +

Positive control: Jurkat or Ramos cells. testicular carcinoma, breast cancer.

References:
- Bebók, Zs, Márkus, B. And Németh, P. Breast Cancer Res.Treatm. 29: 229-235 (1994)