TGF-alpha (Transforming Growth Factor alpha) (clone TG86), anti-human, rabbit, zebrafish

TGF-alpha (Transforming Growth Factor alpha) (clone TG86), anti-human, rabbit, zebrafish

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In stock
SKU
ARP-24-7039-MSM3
Catalog Number: 24-7039-MSM3
Size: 100 µl, 0.5 ml, 1 ml
Isotype: Mouse IgG1, kappa
Decription:
This antibody reacts with the TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. The staining with this monoclonal antibody is completely blocked by the peptide used for raising this antibody. TGFĪ � (aa50) is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.

Synonyms: EGF-like TGF, ETGF, TFGA, TGF Type 1, TGFA, Wa1, Waved 1

Clone: TG86

Isotype: Mouse IgG1, kappa

Immunogen: A 10-amino acid synthetic peptide (aa34-43; PPVAAAVVSH) from human TGFα.

Reactivity: human, rabbit and zebrafish. Others-not known.

Form: Purified, in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Applications: Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (1-2ug/ml)
Immunohistology (Formalin-paraffin) (2-4ug/ml for 30 min at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Reactivity: Jurkat or Ramos cells. Testicular Carcinoma

Concentration: 0.2mg/ml

Storage: Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles.
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