TGF-β1 monoclonal, anti-human
€0.00
In stock
SKU
AC-ABG10540
Catalog Number: AC-ABG10540
Size(s): 500 µg
Host: Mouse
Applications: WB, IHC, E
Datasheet
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Size(s): 500 µg
Host: Mouse
Applications: WB, IHC, E
Datasheet
Request Information
Source:
Produced in mice using highly pure recombinant human TGF-beta1 as the immunizing antigen. This IgG1k antibody was purified from cell culture by Protein G affinity chromatography.
Western Blot:
To detect Human TGF-beta1 by Western Blot analysis this antibody can be used at a concentration of 2.0-4.0 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human TGF-beta1 is 2.0-4.0 ng/lane, under non-reducing conditions.
In a dot blot, a 1:500 dilution of this antibody exhibited complete cross reactivity with Human TGF-beta2 and TGF-beta3 isotypes. Reduced cross reactivity was shown at a 1:1000 dilution.
Sandwich ELISA:
In a sandwich ELISA (assuming 100 μl/well), a concentration of 4.0-8.0 μg/ml of this antibody will detect at least 100 pg/ml of recombinant Human TGF-beta1 when used in conjunction with compatible secondary reagents.
Immunohistochemistry:
This antibody stained formalin-fixed paraffin-embedded sections of human pancreas adenocarcinoma tissue. The recommended concentration is 5.0 μg/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with an alcohol-soluble AEC chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute.
*Additional Immunostaining data available. Please contact Tech Support for information.
Formulation: A sterile filtered antibody solution was lyophilized from PBS.
Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
Produced in mice using highly pure recombinant human TGF-beta1 as the immunizing antigen. This IgG1k antibody was purified from cell culture by Protein G affinity chromatography.
Western Blot:
To detect Human TGF-beta1 by Western Blot analysis this antibody can be used at a concentration of 2.0-4.0 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human TGF-beta1 is 2.0-4.0 ng/lane, under non-reducing conditions.
In a dot blot, a 1:500 dilution of this antibody exhibited complete cross reactivity with Human TGF-beta2 and TGF-beta3 isotypes. Reduced cross reactivity was shown at a 1:1000 dilution.
Sandwich ELISA:
In a sandwich ELISA (assuming 100 μl/well), a concentration of 4.0-8.0 μg/ml of this antibody will detect at least 100 pg/ml of recombinant Human TGF-beta1 when used in conjunction with compatible secondary reagents.
Immunohistochemistry:
This antibody stained formalin-fixed paraffin-embedded sections of human pancreas adenocarcinoma tissue. The recommended concentration is 5.0 μg/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with an alcohol-soluble AEC chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute.
*Additional Immunostaining data available. Please contact Tech Support for information.
Formulation: A sterile filtered antibody solution was lyophilized from PBS.
Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
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