Tim-3 (clone TI 339H), anti-mouse

Decription:
Tim-3 is a Th1-specific cell surface protein. It is expressed selectively by differentiated CD4+Th1 and CD8+Tc1 cells, but is absent on CD4+Th2 and CD8+ Tc2 cells. The Tim gene family has an important role in the regulation of autoimmunity and allergies.

Synonyms: T Cell Immunoglobulin and Mucin Domain Containing Protein-3, HAVcr-2, Hepatitis A Virus Cellular Receptor 2 Homolog

Clone: TI 339H

Isotype: Rat IgG2a kappa

Immunogen: Recombinant mouse Tim-3.

Reactivity: mouse

Specificity: Recognizes mouse Tim-3.

Form: Purified, in 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives.

Applications:
ELISA: (direct or indirect: 1:2'000-1:10'000)
Western blot: (1:2'000-1:10'000 using ECL. Suggested blocking and dilution buffer is TBST with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at rooom temperature).

Working Dilution: Optimal conditions must be determined individually for each application.

Concentration: 1mg/ml

Storage: 2-8C immediate use, or -20C (aliquote)