Tn antigen (clone BRIC111) FITC, anti-human

Decription:
Mouse monoclonal antibody to human Tn Antigen FITC conjugate. Tn is a cryptantigen. It is defined as the core structure of O- glycans (aGalNAc-0-Ser/Thr) but different antibodies may require a contribution to the antigen structure from sialic acid substitution and/or adjacent polypeptide. The antigen is not expressed on normal haemopoietic cells but becomes exposed as a result of an acquired deficiency of b1,3 D-galactosyl transferase. Exposure of Tn antigen on haemopoietic cells is associated with polyagglutination2 and, in some cases thrombocytopenia and/or leucopenia. Detection of the antigen prior to the onset of leukemia has been reported3,4. The Tn antigen has also been described as a useful marker for some types of cancer cells.

Synonyms: Tn Antigen,Tn protein, Cryptantigen

Clone: BRIC111

Isotype: Mouse IgG1 kappa light chain

Immunogen: Tn Antigen. BRIC111 was made in response to Tn positive erythrocytes. It is a direct agglutinin of Tn erythrocytes but unreactive with normal erythrocytes including Group A1 erythrocytes.

Reactivity: human

Form: FITC-conjugate, in PBS buffer, pH 7.2 containing 0.1% sodium azide, 1% BSA

Applications: Immunofluorescence, Flow Cytometry

Working Dilution: 2ul per test (may change from lot to lot)

Storage: 2-8C, do not freeze

References:
1. Cartron, JP., et al, (1978), Eur. J. Biochem. 92, 111-119.
2. Beck, ML., et al, (1977), Med. Lab. Sci. 34, 325-329.
3. Baldwin, ML., et al, (1979) Amer. J. Clin. Path. 72, 1024-1027.
4. Roxby, DJ, et al, (1987) Br. J. Haematol. 67, 153-156.
5. King, MJ., et al, (1991) Transfusion, 31, 142-149.
6. Chester MA et al (ed) (1990) Proceedings of the second international workshop and symposium on monoclonal antibodies against human red blood cells and related antigens, Lund 1990
7. Brooks S.A et al (1995) Expression of alph-GalNAc glycoproteins by breast cancers. Bristish J. of Cancer 71, 1033-1038