TNF-α monoclonal, anti-human

Source:
Produced in BALB/c mice using highly pure recombinant human TNF-alpha as the immunizing antigen. This antibody was purified from cell culture by Protein G affinity chromatography.

Western Blot:
To detect Human TNF-alpha by Western Blot analysis this antibody can be used at a concentration of 0.5-1.0 μg/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant Human TNF-alpha is 0.5-1.0 ng/lane, under reducing conditions and 0.25-0.50 ng/lane, under non-reducing conditions.

Sandwich ELISA:
In a sandwich ELISA (assuming 100 μl/well), a concentration of 2.0-4.0 μg/ml of this antibody will detect at least 1000 pg/ml of recombinant Human TNF-alpha when used with BioGems biotinylated antigen affinity purified anti-Human TNF-alpha (60-325BT) as the detection antibody at a concentration of approximately 0.5-1.0 μg/ml.

Immunohistochemistry:
This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentrations are 15.0-20.0 μg/ml overnight at 4˚C. An HRP-labeled polymer detection system was used with DAB Chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute.
*Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute.

Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of Human TNF-alpha (0.5 ng/ml), a concentration of < 0.1 μg/ml of this antibody is required.

Formulation: A sterile filtered antibody solution was lyophilized from PBS.

Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.