TNF-alpha (clone EBS-O-174), anti-human, rat

Source:
A BALB/c mouse was immunized with human rTNF-alpha.
Fusion partner: NS-1.

Specifications:
EBS-O-174 reacts with human and rat TNF-alpha but not with mouse TNF-alpha. This antibody neutralizes TNF alpha biological activities. It prevents TNF alpha induced apoptosis in Jurkat cells. It also neutralizes Hu-rTNFa-mediated cytotoxicity of L929 cells and inhibits tumor growth in mice. It protects mice against toxicity of Hu-rTNFa.

Species reactivity:
Positive: human, rat. Negative: mouse.

Applications:
Detection of TNF-alpha and in vivo studies.
Blocking function
ELISA: +
Flow cytometry: +
Frozen sections: +
Paraffin sections: -
Western blotting: +

Positive control: HeLa, HL-60, or A431 cells. Macrophages in lymph node or tonsil.

References:
- McLaughlin PJ, et al, Anticancer Res12, 1243-1246 (1992).
- P. Vassalli, Annu Rev Immunol 10: 411-452 (1992).
- M. Pasparakis, et al, Cytokine and Growth Factor Reviews 7: 223-229 (1996).
- Eigler, et al, Immunology Today18: 487-492 (1997).
- Kalli K R, Kaufmann S H, et al.Molecular Pharmacology64(6): 1434-1443 (2003).
- R. Pijnenborg, et al, Placenta19(4): 231-239 (1998).