TNF-alpha human active recombinant protein
€295.00
In stock
SKU
600173
Protein Family: Cytokines
Pathway and Disease: Signal Transduction, Signaling Molecules and Interaction, Cell Growth and Death, Endocrine System, Immune System, Metabolic Disorders
Alternate Names: tumor necrosis, TNF- alpha, tumor necrosis factor ligand superfamily member 2, TNF-a, cachectin, tumor necrosis factor, membrane form, tumor necrosis factor, soluble formDescription:
TNF (Tumor necrosis factor) is secreted by macrophages, monocytes, neutrophils, T-cells, and NK-cells following their stimulation by bacterial LPS. Cells expressing CD4 secrete TNF-alpha while CD8 cells secrete little or no TNF-alpha. The synthesis of TNF-alpha is induced by many different stimuli including interferons, IL2, and GM-CSF. Genetic variations in TNF are associated with susceptibility to psoriasis and hepatitis B virus infection. Recombinant Human Tumor Necrosis Factor-alpha produced in E. coli is a single non-glycosylated polypeptide chain containing 158 amino acids with a MW of 17,483.77 Da.
Source: E. coli. Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.
Applications: E, WB
Application Notes:
Recombinant Tumor Necrosis Factor-alpha is fully biologically active when compared to standard. The ED50 as determined by the cytolysis of mouse L929 cells in the presence of Actinomycin D is less than 0.05 ng/ml.
Accession No.: P01375
MW: 17484 Da
Sequence:
VRSSSRTPSD KPVAHVVANP QAEGQLQWLN RRANALLANG VELRDNQLVV PSEGLYLIYS QVLFKGQGCP STHVLLTHTI SRIAVSYQTK VNLLSAIKSP CQRETPEGAE AKPWYEPIYL GGVFQLEKGD RLSAEINRPD YLDFAESGQV YFGIIAL
Purity:
Purity > 98% as determined by RP-HPLC and reducing and non-reducing SDS-PAGE. Protein Content determined by UV spectroscopy at 280 nm and analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.
Format:
Each vial contains 50 ug protein lyophilized in 10 mM sodium phosphate pH8.0, 20 mM NaCl. Reconstitute with 0.5 ml sterile deionized water for a final concentration of 0.1 mg/ml.
Storage: Store at -20°C. Product is guaranteed one year from the date of shipment. Following reconstitution, store at -20°C. We recommend to add a carrier protein (0.1% HSA or BSA) for long term storage.
Pathway and Disease: Signal Transduction, Signaling Molecules and Interaction, Cell Growth and Death, Endocrine System, Immune System, Metabolic Disorders
Alternate Names: tumor necrosis, TNF- alpha, tumor necrosis factor ligand superfamily member 2, TNF-a, cachectin, tumor necrosis factor, membrane form, tumor necrosis factor, soluble formDescription:
TNF (Tumor necrosis factor) is secreted by macrophages, monocytes, neutrophils, T-cells, and NK-cells following their stimulation by bacterial LPS. Cells expressing CD4 secrete TNF-alpha while CD8 cells secrete little or no TNF-alpha. The synthesis of TNF-alpha is induced by many different stimuli including interferons, IL2, and GM-CSF. Genetic variations in TNF are associated with susceptibility to psoriasis and hepatitis B virus infection. Recombinant Human Tumor Necrosis Factor-alpha produced in E. coli is a single non-glycosylated polypeptide chain containing 158 amino acids with a MW of 17,483.77 Da.
Source: E. coli. Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.
Applications: E, WB
Application Notes:
Recombinant Tumor Necrosis Factor-alpha is fully biologically active when compared to standard. The ED50 as determined by the cytolysis of mouse L929 cells in the presence of Actinomycin D is less than 0.05 ng/ml.
Accession No.: P01375
MW: 17484 Da
Sequence:
VRSSSRTPSD KPVAHVVANP QAEGQLQWLN RRANALLANG VELRDNQLVV PSEGLYLIYS QVLFKGQGCP STHVLLTHTI SRIAVSYQTK VNLLSAIKSP CQRETPEGAE AKPWYEPIYL GGVFQLEKGD RLSAEINRPD YLDFAESGQV YFGIIAL
Purity:
Purity > 98% as determined by RP-HPLC and reducing and non-reducing SDS-PAGE. Protein Content determined by UV spectroscopy at 280 nm and analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.
Format:
Each vial contains 50 ug protein lyophilized in 10 mM sodium phosphate pH8.0, 20 mM NaCl. Reconstitute with 0.5 ml sterile deionized water for a final concentration of 0.1 mg/ml.
Storage: Store at -20°C. Product is guaranteed one year from the date of shipment. Following reconstitution, store at -20°C. We recommend to add a carrier protein (0.1% HSA or BSA) for long term storage.
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