TOP1 monoclonal (N-term), anti-human
€0.00
In stock
SKU
AC-AM2253a
Catalog Number: AC-AM2253a
Size: 80 µl, 400 µl
Isotype: mouse IgG1,κ
Clone Name: 1291CT875.142.166
Applications: FC, WB
Datasheet
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Size: 80 µl, 400 µl
Isotype: mouse IgG1,κ
Clone Name: 1291CT875.142.166
Applications: FC, WB
Datasheet
Request Information
Background:
Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells.
Other Names:
DNA topoisomerase 1, DNA topoisomerase I, TOP1
Target/Specificity:
This TOP1 antibody is generated from a mouse immunized with a recombinant protein from the N-terminal region of human TOP1.
Antigen Type: Recombinant Protein
Gene Name: TOP1
Gene ID: 7150
Primary Accession: P11387
Format: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells.
Other Names:
DNA topoisomerase 1, DNA topoisomerase I, TOP1
Target/Specificity:
This TOP1 antibody is generated from a mouse immunized with a recombinant protein from the N-terminal region of human TOP1.
Antigen Type: Recombinant Protein
Gene Name: TOP1
Gene ID: 7150
Primary Accession: P11387
Format: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
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