Total Cholesterol and Cholesteryl Ester Fluorometric Assay Kit

Detection Principle:
Total Cholesterol (TC) includes free cholesterol (FC) and cholesteryl esters (CE). Cholesterol ester can be hydrolyzed by cholesterol esterase to produce cholesterol and free fatty acid. Cholesterol is oxidized by cholesterol oxidase to produce △4-cholestenone and hydrogen peroxide. In the presence of the enzyme and probe, hydrogen peroxide can be catalyzed to produce the fluorescence substrate. The fluorescence intensity at the excitation wavelength of 535 nm and emission wavelength of 590 nm is proportional to the cholesterol concentration.
Instrument: Fluorescence Microplate Reader
Assay Time: 20 min

Tested Sample Types: Serum, plasma, animal tissue, cells

Research Area's: Lipids metabolism

Shelf Life: 6 Months

Storage:
-20℃