Total Glutathione (T-GSH)/Oxidized Glutathione (GSSG) Colorimetric Assay Kit

Detection Principle:
GSSG is reduced to GSH by glutathione reductase, and GSH can react with DTNB to produce GSSG and yellow TNB. The amount of total glutathione (GSSG+GSH) determines the amount of yellow TNB. Thus the total glutathione can be calculated by measuring the OD value at 412 nm. The content of GSSG can be determined by first removing GSH from the sample with appropriate reagent and then using the above reaction principle.
Instrument: Spectrophotometer
Assay Time: 180 min

Tested Sample Types: Serum,plasma,animal tissue,red blood cells,cultured cells

Research Area's: Oxidative stress

Shelf Life: 6 Months

Storage:
See the manual

Citations:


Detection Principle: http://nopr.niscair.res.in/handle/123456789/44593
https://www.sciencedirect.com/science/article/abs/pii/S0308814621004301