Total Superoxide Dismutase (T-SOD) Activity Assay Kit (WST-1 Method)

Detection Principle:
The activity of SOD was measured by WST-1 method in this kit and the principles of the WST-1 is as follows. Xanthine Oxidase (XO) can catalyze WST-1 react with O2.- to generate a water-soluble formazan dye. SOD can catalyze the disproportionation of superoxide anions, so the reaction can be inhibited by SOD, and the activity of SOD is negatively correlated with the amount of formazan dye. Therefore, the activity of SOD can be determined by the colorimetric analysis of WST-1 products.
Instrument: Microplate reader
Assay Time: 30 min

Tested Sample Types: Serum,plasma,hydrothorax,ascites,urine,cells,tissue

Research Area's: Enzymes,Oxidative stress

Shelf Life: 12 Months

Storage:
Reagent3:-20℃;Others:2-8℃

Citations:


Detection Principle: http://www.besjournal.com/Articles/Archive/2018/No10/201811/t20181112_197101.html
https://www.sciencedirect.com/science/article/pii/S0014299919300822
https://www.sciencedirect.com/science/article/pii/S0031938418306565
https://www.sciencedirect.com/science/article/pii/S0169131718304526
https://www.researchgate.net/profile/Zainab_Al_Mousawi/publication/331843213
https://www.hindawi.com/journals/jdr/2018/7341242/abs/
https://www_mdpi.gg363.site/2218-273X/9/9/453
https://link_springer.gg363.site/article/10.1007/s12640-019-00040-y
https://doi.org/10.1016/j.biopha.2020.110138
https://pubmed.ncbi.nlm.nih.gov/32149046/
https://pubmed.ncbi.nlm.nih.gov/31883161/
https://pubmed.ncbi.nlm.nih.gov/32179202/
https://pubmed.ncbi.nlm.nih.gov/32339907/
https://www.researchgate.net/profile/Yahya_Shekha2/publication/337951595
https://www.researchgate.net/profile/Banankh_Al-Baggou/publication/331049437
https://www.researchgate.net/profile/Abdulkareem_Jewad/publication/337783411
https://edusj.mosuljournals.com/article_164395.html
http://www.academia.edu/download/61575795/974F4AE6253220191221-40278-19yl6pj.pdf
http://pvj.com.pk/in_press/18-400.pdf