TRANCE/RANK L/TNFSF11 human recombinant protein

Background:
TNFSF11, also known as RANKL, is a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. TNFSF11 also has a function in the immune system, where it is expressed by T helper cells and is thought to be involved in dendritic cell maturation. TNFSF11 is important in bone metabolism. This natural and necessary surface-bound molecule (also known as CD254) found on osteoblasts serves to activate osteoclasts, which are the cells involved in bone resorption. Recombinant human TNFSF11 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Alternative Names:
Tumor necrosis factor ligand superfamily member 11, Osteoclast differentiation factor, ODF, Osteoprotegerin ligand, OPGL, Receptor activator of nuclear factor kappa-B ligand, RANKL, TNF-related activation-induced cytokine, TRANCE, CD254

Domain: 140-317aa
Sequence:
IRAEKAMVD GSWLDLAKRS KLEAQPFAHL TINATDIPSG SHKVSLSSWY HDRGWAKISN MTFSNGKLIV NQDGFYYLYA NICFRHHETS GDLATEYLQL MVYVTKTSIK IPSSHTLMKG GSTKYWSGNS EFHFYSINVG GFFKLRSGEE ISIEVSNPSL LDPDQDATYF GAFKVRDID

UniProt No.: O14788
NCBI Accession No.: NP_003692.1

Molecular Weight: 22.3 kDa (199aa) confirmed by MALDI-TOF
Concentration: 0.5mg/ml (determined by Bradford assay)
Formulation: Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT
Purity: > 85% by SDS-PAGE
Bioactivity: Measured by its ability to induce osteoclast differentiation of RAW 264.7 mouse monocyte/macrophage cells. The ED50 range ≤ 25 ng/ml.
Endotoxin: < 1 EU per 1ug of protein (determined by LAL method)
Endotoxin: His-Tag