Transglutaminase II (TGM2) (clone SPM592), anti-human, mouse, rat, rabbit, monkey
€0.00
In stock
SKU
ARP-24-7052-MSM1X
Catalog Number: 24-7052-MSM1X
Size: 100 µl, 0.5 ml, 1 ml
Isotype: Mouse IgG2a, kappa
Size: 100 µl, 0.5 ml, 1 ml
Isotype: Mouse IgG2a, kappa
Decription:
Recognizes a 77-85kDa protein, identified as cellular or tissue transglutaminase II (TGase II). Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene acts as a monomer, is induced by retinoic acid, and appears to be involved in apoptosis. Finally, the encoded protein is the autoantigen implicated in celiac disease. The identification of transglutaminase as the main antigen of endomysium antibodies allows a new diagnostic approach to celiac disease (CD), a genetic, immunologically mediated small bowel enteropathy that causes malabsorption. TGase II is implicated in programmed cell death, signal transduction, drug-resistance, cell growth, endocytosis, insulin secretion, cell adhesion, cataract formation, and wound healing.
Synonyms: G alpha h, G[a]h, GNAH, Guanine nucleotide binding protein H polypeptide, Protein-glutamine gamma-glutamyltransferase 2, TG(C), TG2, TGase C, TGase H, TGase-2, TgaseII, TGC, TGM2, Tissue type transglutaminase, Transglutaminase 2 C polypeptide, Transglutaminase C, Transglutaminase H, Transglutaminase-2, tTG, tTGas
Clone: SPM592
Isotype: Mouse IgG2a, kappa
Immunogen: Recombinant full-length human TGM2 protein
Reactivity: human, monkey, rabbit, mouse and rat. Others not known.
Form: Purified, in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Applications: Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (0.5-1ug/ml)
Immunohistology (Formalin-fixed) (0.5-1ug/ml
30 min at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
Reactivity: HUVEC Cells. Endothelial cells in placenta, liver, brain, or breast carcinoma. Smooth muscle cells of any origin (e.g. intestine)
Concentration: 0.2mg/ml
Storage: Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles.
Recognizes a 77-85kDa protein, identified as cellular or tissue transglutaminase II (TGase II). Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene acts as a monomer, is induced by retinoic acid, and appears to be involved in apoptosis. Finally, the encoded protein is the autoantigen implicated in celiac disease. The identification of transglutaminase as the main antigen of endomysium antibodies allows a new diagnostic approach to celiac disease (CD), a genetic, immunologically mediated small bowel enteropathy that causes malabsorption. TGase II is implicated in programmed cell death, signal transduction, drug-resistance, cell growth, endocytosis, insulin secretion, cell adhesion, cataract formation, and wound healing.
Synonyms: G alpha h, G[a]h, GNAH, Guanine nucleotide binding protein H polypeptide, Protein-glutamine gamma-glutamyltransferase 2, TG(C), TG2, TGase C, TGase H, TGase-2, TgaseII, TGC, TGM2, Tissue type transglutaminase, Transglutaminase 2 C polypeptide, Transglutaminase C, Transglutaminase H, Transglutaminase-2, tTG, tTGas
Clone: SPM592
Isotype: Mouse IgG2a, kappa
Immunogen: Recombinant full-length human TGM2 protein
Reactivity: human, monkey, rabbit, mouse and rat. Others not known.
Form: Purified, in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Applications: Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (0.5-1ug/ml)
Immunohistology (Formalin-fixed) (0.5-1ug/ml
30 min at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
Reactivity: HUVEC Cells. Endothelial cells in placenta, liver, brain, or breast carcinoma. Smooth muscle cells of any origin (e.g. intestine)
Concentration: 0.2mg/ml
Storage: Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles.
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