Triglyceride (TG) Colorimetric Assay Kit (Single Reagent, GPO-PAP Method)

Detection Principle:
Triglycerides (TG) can be hydrolyzed by lipoprotein lipase into glycerol and free fatty acids. Glycerol produces glycerol-3-phosphate and ADP under the catalysis of glycerol kinase (GK). Glycerol-3-phosphate produces hydrogen peroxide under the action of glycerol phosphate oxidase (GPO). In the presence of 4-aminoantipyrine and phenol, hydrogen peroxide is catalyzed by peroxidase (POD) to produce quinones which is proportional to the content of TG.
Instrument: Microplate reader
Assay Time: 30 min

Tested Sample Types: Serum, plasma, cells and tissue

Research Area's: Lipids metabolism

Shelf Life: 6 Months

Storage:
2-8℃

Citations:


Detection Principle: http://nopr.niscair.res.in/handle/123456789/44593
https://www.sciencedirect.com/science/article/abs/pii/S1043661819308473?via%3Dihub
http://xueshu.baidu.com/usercenter/paper/show?paperid=1b6r0xe0kc5c0cr0t9270aq0s1431613&site=xueshu_se
https://ps.tbzmed.ac.ir/Article/Pharm-33016
https://www.researchgate.net/profile/Luthfia_Dewi4/publication/338670710
https://pubmed.ncbi.nlm.nih.gov/32678498/
https://pubmed.ncbi.nlm.nih.gov/33022138/
https://pubmed.ncbi.nlm.nih.gov/32643556/
https://www.sciencedirect.com/science/article/pii/S0223523421002373?via%3Dihub
https://link.springer.com/article/10.1007/s12038-021-00178-y