TRITC (clone NI 308), anti-

Background:
The reactivity of the antiserum is directed to TRITC as tested in direct binding enzyme immunoassay, ELISA and immunoblotting. To enhance the specific signal obtained with a monoclonal antibody or a polyclonal second antibody conjugated to TRITC. The phenomenon of a weak reaction of a monoclonal antibody is e.g. well known in different analysis of vital peripheral blood mononuclear cells in suspensions by the expression of surface markers. A similar situation exists in solid phase assay systems (ELISA, blotting, DIBA) when used for the identification and /or quantitative determination of minute amounts of soluble specific antigens or antibodies. The sensitivity of the TRITC hapten-anti-hapten system makes it a valuable alternative to the biotin-avidin system. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:510 to 1:40; in ELISA from 1:100 upwards; in Western blotting from 1:200 upwards. These data should be interpreted as general recommendations only.

Clone: NI 308

Isotype: Mouse IgG1

Source: Highly purified tetramethylrhodamine isothiocyanate isomer R.

Reactivity: Not applicable. This anti-serum has not been tested for cross-reactivity with other fluorescent compounds.

Specificity: Monoclonal Mouse antibody to tetramethylrhodamine isothiocyanate isomer 1

Form: Delipidated, heat inactivated, lyophilized, stable whole ascites. IgG concentration is 1.0 mg/ml. No foreign proteins added.

Applications: Indirect immunofluorescence, ELISA

Storage: Store at 4C, or in small aliquots at -20C.