TrkB (Phospho-Y817) polyclonal, anti-human, mouse, rat

Background:
The Trk proto-oncogene encodes a tyrosine protein kinase, Trk A, also designated Trk gp140, that serves as a receptor for certain neurotrophic factors including nerve growth factor (NGF) and neurotrophin-3 (NT-3). Trk B is a tyrosine kinase gene highly related to Trk A. Trk B expression is confined to tissues within the central and peripheral nervous systems. The brain-derived neurotrophic factor (BDNF) and NT-3, but not NGF, can induce rapid phosphorylation on tyrosine of Trk B gp145, one of the receptors encoded by NTRK2, although BDNF elicits a response at least two orders of magnitude greater than NT-3. Thus it appears that Trk B gp145 may represent a neurotrophic receptor for BDNF and NT-3. The third member of the Trk family of tyrosine kinases, Trk C, encodes a protein designated Trk C gp145 that is preferentially expressed in brain tissue, is equally related to Trk A and Trk B and is a functional receptor for NT-3.

Alternative Name:
Trk-B, NTRK2,

Application Dilution:
WB:1:1,000-1:5,000
ICC:1:50-1:200
IHC:1:50-1:200

Specificity: TrkB (Phospho-Y817) polyclonal antibody detects endogenous levels of TrkB protein only when phosphorylated at Y817.

Immunogen:
Synthetic phospho-peptide corresponding to residues surrounding Tyr817 of human TrkB.

MW: 145 kDa

Swiss-Prot: Q16620(Human) P15209(Mouse) Q63604(Rat)

Purification & Purity:
ProA affinity purified

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

For research use only, not for use in diagnostic procedure.