Tubulin (alpha subunit) (clone TU-02), anti-human, mouse, rat, pig, plants (a.o. Nicotiana tabacum)

Source:
A BALB/c mouse was immunized with microtubular protein from pig brain.

Specifications:
TU-02 reacts with the N-terminal domain of alpha-tubulin. Tubulin isotypes have been identified with tissue specific expression. Immunocytochemical studies using several mAbs revealed remarkable heterogeneity of tubulin within various nervous tissues. TU-02 reacts with tubulin in fixed tissues only, not in unfixed or live tissues or cells. Interphase microtubules are also stained by TU-02 in fixed tissues.

Species reactivity:
Positive: human, mouse, pig, plants (a.o. Nicotiana tabacum), rat.

Applications:
Besides for nerve staining, TU-02 can be applied for a large number of human epithelial and endothelial tissues. It is strongly reactive with the granular cell layer, weakly with the basal and the squamous layer and unreactive with the horny layer of the epidermis or dermis. TU-02 is weakly reactive with connective tissue cells in the dermis and moderately reactive which endothelial cells of blood vessels. It shows strong activity with the epithelium of the trachea and large bronchi, with pancreas and bladder.
ELISA: +
Frozen sections: +
Immunofluorescence: -
Paraffin sections: -
Western blot: -

Positive control: Pancreas, bladder, skin, brain.

References:
- Dráber, P. et. al. Eur.J.Cell.Biol.41: 82-88 (1986).
- Dráber, P. et. al. Histochemistry 87: 151-155 (1987).
- Dráber, P. et. al. J. Cell Science92: 519-528 (1989).
- Smertenko et al. Eur. J. Cell. Biol.72: 104-112 (1997).