Tubulin (Bovine Brain) Lysate
€179.00
In stock
SKU
ECM-TL7151
Background:
Microtubules (MTs) are cytoskeletal elements that play an essential role in cell division and cytoplasmic organization. MTs are dynamic polymers of a/β-Tubulin heterodimers. At least two populations of MTs, called dynamic and stable according to their rates of turnover, are readily distinguishable in cells. The proteins associated with MTs (MAPs) are among the best-known factors that regulate MT dynamics and stability. For preparation of tubulin lysate, bovine brain tubulin was purified and quantified using the BCA method (Pierce) before diluting to the final concentration in gel electrophoresis sample buffer.
Buffer/Storage:
Tubulin Lysate is supplied at a concentration of 20 ng/µl in electrophoresis sample buffer (62.5 mM Tris pH 6.8, 2% SDS, 5% glycerol, 0.003% bromophenol blue, 0.9% β-mercaptoethanol). Store at –20°C. Do not boil or dilute. Stable for 1 year.
Microtubules (MTs) are cytoskeletal elements that play an essential role in cell division and cytoplasmic organization. MTs are dynamic polymers of a/β-Tubulin heterodimers. At least two populations of MTs, called dynamic and stable according to their rates of turnover, are readily distinguishable in cells. The proteins associated with MTs (MAPs) are among the best-known factors that regulate MT dynamics and stability. For preparation of tubulin lysate, bovine brain tubulin was purified and quantified using the BCA method (Pierce) before diluting to the final concentration in gel electrophoresis sample buffer.
Buffer/Storage:
Tubulin Lysate is supplied at a concentration of 20 ng/µl in electrophoresis sample buffer (62.5 mM Tris pH 6.8, 2% SDS, 5% glycerol, 0.003% bromophenol blue, 0.9% β-mercaptoethanol). Store at –20°C. Do not boil or dilute. Stable for 1 year.
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