UBE2D2 polyclonal, anti-human, mouse, rat

UBE2D2 polyclonal, anti-human, mouse, rat

€305.00
In stock
SKU
BS5969
Catalog Number: BS5969
Size: 50 ul, 100 ul
Isotype: rabbit IgG
Applications: WB, ICC

Datasheet
Background:
Rub1 shares 53% homology with ubiquitin and requires activation via the E2 proteins, including Ula1, Uba3 and Ubc12, in order to conjugate to substrates directed to different proteolytic systems. UBE2D2 catalyzes ubiquitination of IkBa in a phosphorylation and SCFB-TRCP dependent manner. In this particular reaction, E1 first transfers ubiquitin to the E2 component UBE2D2, and UBE2D2 then associates with E3 ligase, which conjugates the poly-ubiquitin chain on a target protein. In this fashion, the chain tags the IkBa for degradation by a proteasome thus lifting the inhibitory effect of IkBa on NFkB and allowing NFkB to enter the nucleus.

Alternative Name:
Ubiquitin-conjugating enzyme E2 D2, Ubiquitin carrier protein D2, Ubiquitin-conjugating enzyme E2(17)KB 2, Ubiquitin-conjugating enzyme E2-17 kDa 2, Ubiquitin-protein ligase D2, p53-regulated ubiquitin-conjugating enzyme 1, PUBC1, UBC4, UBC5B, UBCH4, UBCH5B

Application Dilution: WB: 1:500~1:1000, ICC: 1:50~1:200

Specificity: UBE2D2 polyclonal antibody detects endogenous levels of UBE2D2 protein.

Immunogen:
Synthetic peptide, corresponding to amino acids 102-147 of Human UBE2D2.

MW: ~ 17 kDa

Swis Prot.: P62837

Purification & Purity:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Format:
1mg/ml in PBS with 0.1% Sodium Azide, 50% Glycerol.

Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

For research use only, not for use in diagnostic procedure.
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