Urea (BUN) Colorimetric Assay Kit (Diacetyl Oxime Method)

Detection Principle:
In strong acidic and heating condition, urea can react with diacetyl to form red diazine compound. The depth of color is proportional to the content of urea. Because the instability of the diacetyl, the diacetyl oxime usually react with the strong acid firstly in the reaction system to generate diacetyl, then react with urea to generate the red diazine compound. The reaction equation is as follows:
Instrument: Spectrophotometer
Assay Time: 60 min

Tested Sample Types: Serum,plasma,saliva,urine

Research Area's: Kidney Biomarkers

Shelf Life: 6 Months

Storage:
2-8℃