Urea (BUN) Colorimetric Assay Kit (Urease Method)

Detection Principle:
Urea can be decomposed into ammonia ion and carbon dioxide by urease. Ammonia ion can react with amphyl and form a green substance in alkaline medium, and the production of the green substance is proportional to the urea content which can be calculated with the colorimetric assay at 580 nm.
Instrument: Microplate reader
Assay Time: 30 min

Tested Sample Types: Serum,plasma,urine,saliva,milk

Research Area's: Kidney Biomarkers

Shelf Life: 6 Months

Storage:
2-8℃

Citations:


Detection Principle: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6771317/
https://www.ncbi.nlm.nih.gov/pubmed/31824338
https://onlinelibrary.wiley.com/doi/10.1002/smll.202006223