VEGF-165 rat active recombinant protein
€295.00
In stock
SKU
600384
Protein Family: CAM Ligands, Cytokines
Pathway and Disease: Cancers, Cell Communication, Signal Transduction, Signaling Molecules and Interaction
Alternate Names: Vascular endothelial growth factor a, VEFG-a, vascular permeability factor, VPF, VEFGA, VEGFDescription:
Vascular Endothelial Growth Factor-A (VEGF-A) was originally isolated from tumor cells and is produced by a wide variety of cell types. In addition to stimulating vascular growth and vascular permeability, VEGF-A may play a role in stimulating vasodilatation via nitric oxide-dependent pathways. VEGF-A has several variants, VEGF-165 being the most abundant. Rat and bovine VEGF are one amino acid shorter than the human factor, and the bovine and human sequences show a homology of 95%. Rat VEGF-165 is produced in E.coli. Recombinant rat VEGF-165 is a disulfide linked homodimer, containing two 165 amino acids chains, with a total molecular weight of 38.8 kDa.
Source: E. coli. Endotoxin level as measured by LAL is <0.05ng/ug or <0.5EU/ug.
Applications: E, WB
Application Notes:
The activity is determined by the dose-dependent induced proliferation of Human umbilical vein endothelial cells (HUVECs) and is typically 4.0-8.0 ng/mL. Specific activity: 2.5 x 10e5 units/mg
Accession No.: P16612-2
MW: 38800 Da
Sequence:
MAPTTEGEQK AHEVVKFMDV YQRSYCRPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCAGC CNDEALECVP TSESNVTMQI MRIKPHQSQH IGEMSFLQHS RCECRPKKDR TKPEKHCEPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR
Purity:
Purity > 95% as determined by RP-HPLC and reducing and non-reducing SDS-PAGE. Protein Content determined by UV spectroscopy at 280 nm and analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.
Format:
Each vial contains 10 ug of lyophilized protein. Reconstitute with 0.1 ml sterile deionized water for a final concentration of 0.1 mg/ml.
Storage: Store at -20°C. Product is guaranteed one year from the date of shipment. Following reconstitution, store at -20°C. We recommend to add a carrier protein (0.1% HSA or BSA) for long term storage.
Pathway and Disease: Cancers, Cell Communication, Signal Transduction, Signaling Molecules and Interaction
Alternate Names: Vascular endothelial growth factor a, VEFG-a, vascular permeability factor, VPF, VEFGA, VEGFDescription:
Vascular Endothelial Growth Factor-A (VEGF-A) was originally isolated from tumor cells and is produced by a wide variety of cell types. In addition to stimulating vascular growth and vascular permeability, VEGF-A may play a role in stimulating vasodilatation via nitric oxide-dependent pathways. VEGF-A has several variants, VEGF-165 being the most abundant. Rat and bovine VEGF are one amino acid shorter than the human factor, and the bovine and human sequences show a homology of 95%. Rat VEGF-165 is produced in E.coli. Recombinant rat VEGF-165 is a disulfide linked homodimer, containing two 165 amino acids chains, with a total molecular weight of 38.8 kDa.
Source: E. coli. Endotoxin level as measured by LAL is <0.05ng/ug or <0.5EU/ug.
Applications: E, WB
Application Notes:
The activity is determined by the dose-dependent induced proliferation of Human umbilical vein endothelial cells (HUVECs) and is typically 4.0-8.0 ng/mL. Specific activity: 2.5 x 10e5 units/mg
Accession No.: P16612-2
MW: 38800 Da
Sequence:
MAPTTEGEQK AHEVVKFMDV YQRSYCRPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCAGC CNDEALECVP TSESNVTMQI MRIKPHQSQH IGEMSFLQHS RCECRPKKDR TKPEKHCEPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR
Purity:
Purity > 95% as determined by RP-HPLC and reducing and non-reducing SDS-PAGE. Protein Content determined by UV spectroscopy at 280 nm and analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.
Format:
Each vial contains 10 ug of lyophilized protein. Reconstitute with 0.1 ml sterile deionized water for a final concentration of 0.1 mg/ml.
Storage: Store at -20°C. Product is guaranteed one year from the date of shipment. Following reconstitution, store at -20°C. We recommend to add a carrier protein (0.1% HSA or BSA) for long term storage.
| Is Featured? | No |
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