VEGF-A (clone VEGF-21), anti- human, mouse, rat, dog, rabbit

Source:
A BALB/c mouse was immunized with human VEGF189 recombinant protein.

Specifications:
VEGF-21 reacts with Vascular Endothelial Growth Factor, also known as Vascular Permeability Factor (VEGF/ VPF) and is the key mediator of angiogenesis. The MWs are 19-22kDa (reducing) and 38kDa-44kDa (non-reducing). There are multiple isoforms of VEGF containing 206-, 189-, 165-, and 121-amino acid residues. The smaller two isoforms, VEGF165 and VEGF121, are secreted proteins and act as diffusible agents, whereas the larger two remain cell associated. VEGF/VPF plays an important role in angiogenesis, which promotes tumor progression and metastasis. In addition to endothelial cells, VEGF and VEGF receptors are expressed on numerous non-endothelial cells including tumor cells.

Species reactivity:
Positive: human, dog, mouse, rabbit, rat.

Applications:
VEGF-21 can be applied for immunohistochemistry on frozen and paraffin sections. Astrocytomas, breast and ovarian carcinomas are positive.
Flow cytometry: +
Frozen sections: +
Immunofluorescence: +
Paraffin sections: + Citrate

Positive control: Astrocytomas, breast or ovarian carcinomas.

References:
-
- Tischer E. et al. J. Biol. Chem 266: 11947-11954 (1991).
- Berse B. et al, Mol. Biol. Cell 3: 211-220 (1992)