VEGF, anti-human monoclonal antibody

Source:
Produced in BALB/c mice using highly pure (>98%) recombinant human VEGF165as the immunizing antigen. This IgG1k antibody was purified from cell culture by Protein A chromatography.

Preparation: To detect hVEGF165 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hVEGF165is 0.25-0.50 ng/lane, under non-reducing conditions.

Sandwich ELISA:
In a sandwich ELISA (assuming 100ul/well), a concentration of 2.0-4.0 µg/ml of this antibody will detect at least 100 pg/ml of recombinant human VEGF165when used with BioGems Biotinylated antigen affinity purified anti-human VEGF165(60-342BT) as the detection antibody at a concentration of approximately 0.25-1.0 µg/ml.

Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of Human VEGF (10 ng/ml), a concentration of ≤;0.2 µg/ml of this antibody is required.

Formulation:
A sterile filtered antibody solution was lyophilized from PBS.

Reconstitution:
Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.