VEGFR1/Flt-1 mouse recombinant protein

Background:
VEGFR1 (Vascular Endothelial Growth Factor Receptor 1), also known as FLT-1 (Fms-like Tyrosine Kinase 1), belongs to the class III subfamily of receptor tyrosine kinases. While VEGFR1, VEGFR2, and VEGFR3 are mainly expressed on endothelial cells, VEGFR1 is uniquely expressed on macrophages and primarily plays inhibitory roles. Inhibitors of VEGFR are used in cancer treatment. VEGFR1 acts as a cell-surface receptor for VEGFA, VEGFB, and PGF, playing essential roles in the development of embryonic vasculature, regulation of angiogenesis, cell survival, cell migration, macrophage function, chemotaxis, and cancer cell invasion. VEGFR1 binds VEGF with higher affinity than VEGFR2 but exhibits weaker kinase activity. The VEGF-kinase ligand/receptor signaling system is crucial for vascular development and the regulation of vascular permeability. Recombinant mouse VEGFR1/Flt-1, fused to hIgG-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Alternative Names:
VEGFR1, VEGF R1, VEGFR-1, FLT-1, FLT1, FLT, sFlt1, Emrk2, FRT, Vascular endothelial growth factor receptor 1 isoform 1, Embryonic receptor kinase 2, Fms-like tyrosine kinase 1, Tyrosine-protein kinase FRT, Tyrosine-protein kinase receptor FLT, Vascular permeability factor receptor, fms-related tyrosine kinase 1

Domain: 23-759aa
Sequence:
YGSGSKLKVP ELSLKGTQHV MQAGQTLFLK CRGEAAHSWS LPTTVSQEDK RLSITPPSAC GRDNRQFCST LTLDTAQANH TGLYTCRYLP TSTSKKKKAE SSIYIFVSDA GSPFIEMHTD IPKLVHMTEG RQLIIPCRVT SPNVTVTLKK FPFDTLTPDG QRITWDSRRG FIIANATYKE IGLLNCEATV NGHLYQTNYL THRQTNTILD VQIRPPSPVR LLHGQTLVLN CTATTELNTR VQMSWNYPGK ATKRASIRQR IDRSHSHNNV FHSVLKINNV ESRDKGLYTC RVKSGSSFQS FNTSVHVYEK GFISVKHRKQ PVQETTAGRR SYRLSMKVKA FPSPEIVWLK DGSPATLKSA RYLVHGYSLI IKDVTTEDAG DYTILLGIKQ SRLFKNLTAT LIVNVKPQIY EKSVSSLPSP PLYPLGSRQV LTCTVYGIPR PTITWLWHPC HHNHSKERYD FCTENEESFI LDPSSNLGNR IESISQRMTV IEGTNKTVST LVVADSQTPG IYSCRAFNKI GTVERNIKFY VTDVPNGFHV SLEKMPAEGE DLKLSCVVNK FLYRDITWIL LRTVNNRTMH HSISKQKMAT TQDYSITLNL VIKNVSLEDS GTYACRARNI YTGEDILRKT EVLVRDSEAP HLLQNLSDYE VSISGSTTLD CQARGVPAPQ ITWFKNNHKI QQEPGIILGP GNSTLFIERV TEEDEGVYRC RATNQKGAVE SAAYLTVQGT SDKSNLE

UniProt No.: P35969
NCBI Accession No.: NP_034358.2

Molecular Weight: 108.9kDa (970aa)
Concentration: 1mg/ml (determined by Absorbance at 280nm)
Formulation: Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Purity: > 85% by SDS-PAGE
Bioactivity: Measured by its ability to inhibit proliferation using HUVEC human umbilical vein endothelial cells in the presence of Human VEGF165. The ED50 range ≤ 50ng/ml.
Endotoxin: < 1 EU per 1ug of protein (determined by LAL method)
Endotoxin: hIgG-His-Tag
Storage: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.