Vimentin (clone EBS-O-184), anti-human

Source:
A BALB/c mouse was immunized with human recombinant full-length human vimentin protein.
Fusion partner: Sp2/0.

Specifications:
EBS-O-184 reacts with vimentin, a 58kDa protein. Anti-vimentin alone is of limited value as a diagnostic tool; however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma.

Species reactivity:
Positive: human.

Applications:
Identification of sarcomas, melanomas, Schwannomas and meningiomas. Differentiation of melanomas from undifferentiated carcinomas, hepatomas, seminomas and large cell lymphomas.Anti-vimentin is also useful as atissue process control reagent.
Flow cytometry: +
Frozen sections: +
Immunofluorescence: +
Paraffin sections: +

Positive control: Jurkat cells, sarcomas, melanomas.

References:
- Stathopoulos, E, et al, J. Histochem. Cytochem.37: 1363-1370 (1989)