Vinculin VCL monoclonal, anti-human, mouse, rat

Description: Anti-Vinculin VCL Rabbit Monoclonal Antibody . Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.

Protein Function:
Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell- surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion..

Other Names: Vinculin, Metavinculin, MV, VCL

Clone Name(s):
Clone: AIG-22

Subcellular Localization: Cytoplasm, cytoskeleton. Cell junction, adherens junction. Cell membrane ; Peripheral membrane protein ; Cytoplasmic side. Cell junction, focal adhesion. Cytoplasmic face of adhesion plaques. Recruitment to cell-cell junctions occurs in a myosin II- dependent manner. Interaction with CTNNB1 is necessary for its localization to the cell-cell junctions (By similarity). Colocalizes with LIMD1 in the focal adhesions..

Tissue Specificity: Metavinculin is muscle-specific.

Gene ID: 7414

Immunogen: A synthesized peptide derived from human Vinculin

Calculated MW: 123799

Purification: Affinity-chromatography

Format: Liquid

Contents: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Concentration (mg/ml): Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage: Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.