Vitamin B1 (VB1) detection kit

Use: Used to detect the concentration of vitamin B1 (VB1) in samples such as serum, plasma, and cell culture supernatant.Specificity: The kit uses enzyme-linked immunosorbent assay. Biotin-labeled vitamin B1 (VB1) is used, and the purified anti-vitamin B1 (VB1) antibody is coated on the microplate. In the competitive inhibition reaction, a certain amount of solid-phase antibody inhibits the competitive reaction with biotin-labeled vitamin B1 (VB1) and non-labeled antigen (calibrator or specimen). The binding amount of the antibody and biotin-labeled vitamin B1 (VB1) is inhibited by the amount of non-labeled antigen. The more non-labeled antigen, the less the antibody and biotin-labeled vitamin B1 (VB1), and vice versa. After the reaction is balanced, a solid-phase antibody-biotinylated vitamin B1 (VB1) is formed, and then enzyme-labeled avidin is added to form a solid-phase antibody-biotinylated vitamin B1 (VB1)-enzyme label-avidin complex. After adding substrate for color development, the absorbance (OD value) is measured at a wavelength of 450nm using an enzyme reader. As the concentration of vitamin B1 (VB1) increases, the OD value gradually decreases and shows a good linear relationship. This kit has the characteristics of high sensitivity, strong specificity, good repeatability, simple operation and rapidity, and has reliable detection performance for the decrease or increase of vitamin B1 (VB1) in samples.

Storage Conditions
Store at 2-8°C.

Use: The kit is for research use only.