Vitamin C (VC) Colorimetric Assay Kit

Detection Principle:
The most obvious chemical activity of VC is that reduce Fe3+ to Fe2+, then promote iron absorption in the intestine, promote the storage and utilization of iron. Fe3+ react immediately with reducing ascorbic acid to form Fe2+. then Fe2+ react with phenanthroline and the color developing reaction occurs. The content of vitamin C in sample can be determined. Measure the OD value and calculate the VC content indirectly.
Instrument: Microplate reader
Assay Time: 50min

Tested Sample Types: Serum,plasma,tissue

Research Area's: Oxidative stress,Plant stress resistance

Shelf Life: 6 Months

Storage:
2-8℃