Wrb antigen (clone BRIC14) Affinity purified, anti-human

Decription:
Mouse monoclonal antibody to human Wrb Antigen Affinity purified. The Wrb antigen is defined as the amino acid sequence at residue 658 (GLU) of the major erythrocyte anion transport protein band 31,2,. The antigen is stabilised by the association between band 3 and Glycophorin A (GPA, CD 235a). It is found only on erythroid cells. There are approximately 106 antigen sites per erythrocyte4. The antigen is found on all human erythrocytes except for the very rare Wr(b-) and Glycophorin A deficient types.

Synonyms: Wrb antigen, Wrb protein

Clone: BRIC14

Isotype: Mouse IgG2a kappa light chain

Immunogen: Wright red blood cells

Reactivity: human

Form: Affinity purified, in 0.1 M glycine buffer, 1 M Tris buffer, pH 7.4, sterile filtered

Applications: ELISA

Working Dilution: Functional binding affinity for erythrocytes of 3.2 x 10^7M 1

Storage: 2-8C for immediate use, or at -20C (aliquot)

References:
1. Paulitschke et al. Blood 1995, 86: 342-348.
2. Bruce et al. Blood 1995, 85: 541-547.
3. Anstee D.J., (1990) Vox Sang. 58, 1-20 (Review)
4. Gardner B. et al (1989) Immunology 68, 283-289
5. Pasvol G. et al. (1989) Blood 74, 1836-1843