ZAP70 (Chronic Lymphocytic Leukemia Marker) (clone 2F3.2 ), anti-human

Background:
ZAP70 is a 70kDa protein tyrosine kinase found in T-cells and natural killer cells. ĀControl of this protein translation is via the IgVH gene.ĀIn Western blotting of whole cell lysates of normal peripheral blood mononuclear cells, the antibody labels a band corresponding to ZAP70. In Western blotting of whole cell lysates of CD19-positive purified leukemia cells from patients with Ig-unmutated and Ig-mutated CLL, the antibody labels a band corresponding to ZAP70 in the Ig-unmutated CLL samples, whereas no band is observed in the Ig-mutated CLL samples. In Western blotting of cell lysates of Jurkat cells (T-lymphoblastic cell line), the antibody labels a band of 70kDa protein. In Western blotting of cell lysates of A431 cells (carcinoma cell line), no band is observed. ZAP70 protein is expressed in leukemic cells of approximately 25% of chronic lymphocytic leukemia (CLL) cases as well. ĀAnti-ZAP70 expression is an excellent surrogate marker for the distinction between the Ig-mutated (anti-ZAP70 negative) and Ig-unmutated (anti-ZAP70 positive) CLL subtypes and can identify patient groups with divergent clinical courses. The anti-ZAP70 positive Ig-unmutated CLL cases have been shown to have a poorer prognosis.

Other Names:
Tyrosine-protein kinase ZAP-70, 2.7.10.2, 70 kDa zeta-chain associated protein, Syk-related tyrosine kinase, ZAP70, SRK

Gene Name: ZAP70
Gene ID: 7535
Primary Accession: P43403
Other Accession: 7535, 234569