C-Raf (C-terminus) Blocking Peptide
€155.00
In stock
SKU
ECM-RX2075
Background:
The Ras-Raf-MAP kinase signaling pathway is involved in control of cell proliferation and differentiation. The Raf kinase family includes A-Raf, B-Raf, and C-Raf. Each family member has three highly conserved regions (CR1-3). The N-terminal CR1 contains the Ras-GTP-binding domain. The CR2 contains a negative regulatory serine residue (C-Raf (S259)/B-Raf(S365)) that may bind 14-3-3 proteins. The CR3 is the catalytic domain that contains phosphorylation sites for Raf-regulating enzymes within two segments, the N-region and the activation segment. Activation of C-Raf involves phosphorylation at many sites including Ser-338, Tyr-341, and multiple catalytic domain sites. EGF receptor activation leads to phosphorylation of Ser-471, which is critical for C-Raf kinase activity and is required for interaction with MEK. In B-Raf, the corresponding conserved site is Ser-578, and mutation of this residue to alanine produces an inactivate kinase. Thus, this Raf phosphorylation site may be critical for kinase activity and may be important for MEK binding and activation.
Background References
Mason, C.S. et al. (1999) EMBOJ 18(8):2137.
Zhu, J. et al. (2005) Mol. Biol. Cell 16:4733.
Sequence: C-Raf (C-terminus) synthetic peptide corresponding to amino acids 637 to 648 in human C-Raf. This sequence is conserved in C-Rafs from many species, and is not found in A-Raf or B-Raf.
Specificity: The peptide is specifically recognized by anti-C-Raf (C-terminus) antibody (RP2071) in ELISA, and has been shown to block the reactivity of RP2071 during Western blot. In addition, the peptide is recommended for use in blocking RP2071 reactivity in immunocytochemistry.
Application dilution:
Blocking: 1:1000
ELISA: 50 ng/well
End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1 hour at room temperature.
Buffer/Storage:
Blocking Peptide is supplied in 50µl phosphate-buffered saline and 0.05% sodium azide. Store at –20°C. Stable for 1 year.
The Ras-Raf-MAP kinase signaling pathway is involved in control of cell proliferation and differentiation. The Raf kinase family includes A-Raf, B-Raf, and C-Raf. Each family member has three highly conserved regions (CR1-3). The N-terminal CR1 contains the Ras-GTP-binding domain. The CR2 contains a negative regulatory serine residue (C-Raf (S259)/B-Raf(S365)) that may bind 14-3-3 proteins. The CR3 is the catalytic domain that contains phosphorylation sites for Raf-regulating enzymes within two segments, the N-region and the activation segment. Activation of C-Raf involves phosphorylation at many sites including Ser-338, Tyr-341, and multiple catalytic domain sites. EGF receptor activation leads to phosphorylation of Ser-471, which is critical for C-Raf kinase activity and is required for interaction with MEK. In B-Raf, the corresponding conserved site is Ser-578, and mutation of this residue to alanine produces an inactivate kinase. Thus, this Raf phosphorylation site may be critical for kinase activity and may be important for MEK binding and activation.
Background References
Mason, C.S. et al. (1999) EMBOJ 18(8):2137.
Zhu, J. et al. (2005) Mol. Biol. Cell 16:4733.
Sequence: C-Raf (C-terminus) synthetic peptide corresponding to amino acids 637 to 648 in human C-Raf. This sequence is conserved in C-Rafs from many species, and is not found in A-Raf or B-Raf.
Specificity: The peptide is specifically recognized by anti-C-Raf (C-terminus) antibody (RP2071) in ELISA, and has been shown to block the reactivity of RP2071 during Western blot. In addition, the peptide is recommended for use in blocking RP2071 reactivity in immunocytochemistry.
Application dilution:
Blocking: 1:1000
ELISA: 50 ng/well
End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1 hour at room temperature.
Buffer/Storage:
Blocking Peptide is supplied in 50µl phosphate-buffered saline and 0.05% sodium azide. Store at –20°C. Stable for 1 year.
| Is Featured? | No |
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