CD4 FITC/CD8 PE/CD3 PerCP (clones 33-2A3, HP2-6, D3/9), anti-human RUO

Introduction:
The CD3 monoclonal antibody is directed against the CD3 antigen (T3-antigen), a component of the T-cell receptor complex expressed on the surface of human T lymphocytes. This antibody reacts with 80–90% of human peripheral T cells and medullary thymocytes. It does not react with B cells, monocytes, granulocytes, or platelets. The CD3 antigen is involved in signal transduction following antigen recognition and is essential for T-cell activation. The antibody may be mitogenic for resting T lymphocytes and can block the cytolytic activity of cytotoxic T lymphocyte (CTL) clones.
The CD4 monoclonal antibody recognizes the CD4 antigen (T4-antigen), a glycoprotein expressed on approximately 60–65% of human peripheral T lymphocytes and on most thymocytes. CD4 is a co-receptor for the T-cell receptor (TCR) that binds to MHC class II molecules, facilitating T-cell activation. The antibody may show weak reactivity with monocytes and macrophages but does not react with B cells, granulocytes, or platelets. CD4 expression defines the helper/inducer T-cell subset, which plays a central role in immune regulation and coordination.
The CD8 monoclonal antibody is directed against the CD8 antigen (T8-antigen), a cell surface glycoprotein expressed on approximately 25–35% of peripheral T lymphocytes and on a subset of thymocytes. CD8 acts as a co-receptor for the TCR complex in recognition of antigens presented by MHC class I molecules. The antibody does not react with B cells, monocytes, granulocytes, or platelets. CD8 expression defines the cytotoxic/suppressor T-cell subset responsible for direct killing of target cells and immune surveillance.
Together, the CD4/CD8/CD3 combination allows for the immunophenotypic identification and enumeration of total T cells and their major functional subsets (helper/inducer and cytotoxic/suppressor T lymphocytes) by flow cytometry. This combination provides valuable information for the evaluation of immune status in both physiological and pathological conditions, including immunodeficiencies, autoimmune disorders, infections, and hematologic malignancies

Recommended Usage:
Immunostep’s CD3/CD4/CD8 is a monoclonal antibody combination intended for immunophenotypic identification and enumeration of T-lymphocyte subpopulations in human peripheral blood or other biological samples. This reagent allows the simultaneous detection of total T cells (CD3⁺), helper/inducer T cells (CD4⁺), and cytotoxic/suppressor T cells (CD8⁺) by flow cytometry. The combined analysis of these markers provides valuable information for the assessment and monitoring of immune status in various clinical conditions, including immunodeficiencies, autoimmune diseases, infectious processes, and hematologic disorders. This reagent is effective for direct immunofluorescence staining of human tissue for flow cytometric analysis using 1 test for 106 cells.

Application: Flow Cytometry

Storage Temperature: Store in the dark at 2-8 °C

Shelf life: Each reagent is stable until the expiration date shown on the bottle label when stored as directed (at least, one year)