CD8a polyclonal, anti-human

Description:
CD8 is a cell surface receptor expressed either as a heterodimer with the CD8 β chain (CD8 α/β) or as a homodimer (CD8 α/α). A majority of thymocytes and a subpopulation of mature T cells and NK cell express CD8a. CD8 binds to MHC class 1 and through its association with protein tyrosine kinase p56lck plays a role in T cell development and activation of mature T cells. For mature T-cells, CD4 and CD8 are mutually exclusive, so anti-CD8, generally used in conjunction with anti-CD4. It is a useful marker for distinguishing helper/inducer T-lymphocytes, and most peripheral T-cell lymphomas are CD4+/CD8-. Anaplastic large cell lymphoma is usually CD4+ and CD8-, and in T-lymphoblastic lymphoma/leukemia, CD4 and CD8 are often co-expressed. CD8 is also found in littoral cell angioma of the spleen.

Synonyms:
CD8 antigen, alpha polypeptide (p32), T8/Leu-2 T-lymphocyte differentiation antigen, Ly3, LYT3, MAL, T-cell surface glycoprotein CD8 alpha chain

Isotype: Rabbit polyclonal IgG

Immunogen:
Human CD8 recombinant fragment corresponding to aa 200-250

Form: Purified, in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Reactivity: human. Others not known.

Applications: Flow Cytometry (1-2ug/million cells in 0.1ml)
Immunofluorescence (1-2ug/ml)
Optimal dilution for a specific application should be determined.

Positive Control:
HuT78 or hPBL. Tonsil

Molecular Weight:
32kDa

Concentration:
0.2mg/ml

Storage: Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles.