CHK2 polyclonal, anti-human

Background:
Cell cycle events are regulated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by proteolysis of cyclins. Chk1 and Chk2 are involved in these processes as regulators of Cdks. Chk1 and Chk2 both function as essential components in the G2 DNA damage checkpoint by phosphorylating Cdc25C in response to DNA damage. Phosphorylation inhibits Cdc25C activity, thereby blocking mitosis. Cdc25A, Cdc25B and Cdc25C protein tyrosine phosphatases function as mitotic activators by dephosphorylating Cdc2 p34 on regulatory tyrosine residues. It has also been shown that Chk1 can phosphorylate Wee1 in vitro, providing evidence that the hyperphosphorylated form of Wee1, seen in cells delayed by Chk1 overexpression, is due to phosphorylation by Chk1.

Alternative Name:
Hucds1, hCds1, CHEK2, CDS 1, Cds1, Cds1 homolog, Checkpoint kinase 2, Checkpoint like protein CHK2, CHEK 2, Chek2, Chk 2, CHK2 checkpoint homolog (S. pombe), CHK2 checkpoint homolog, CHK2, hCds1, HuCds 1, LFS 2, LFS2, PP1425, RAD 53, RAD53, Rad53 homolog, Serine/threonine protein kinase Chk2, Serine/threonine-protein kinase Chk2,

Application Dilution:
WB:1:1,000-1:2,000
ICC:1:50-1:200
IHC:1:50-1:200

Specificity: CHK2 polyclonal antibody detects endogenous levels of CHK2 protein.

Immunogen:
recombinant protein

MW: 62 kDa

Swiss-Prot: O96017(Human)

Purification & Purity:
ProA affinity purified

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

For research use only, not for use in diagnostic procedure.