Coronin-1B (phospho-Ser-2) Blocking Peptide
€155.00
In stock
SKU
ECM-CX2625
Background:
Coronins are highly-conserved F-actin binding proteins that play important roles in lamellipodial protrusion during various types of cell motility. In yeast, coronins regulate cytoskeletal changes through inhibition of Arp2/3 complex. Human coronins have been classified in three subgroups type I (coronin-1A, -1B, -1C), type II (coronin-2A, -2B), and type III (coronin-7). These coronins have at least one large b-propeller region that mediates protein-protein interactions and type I and II coronins have coiled-coil regions involved in oligiomerization. Coronin-1B is ubiquitously expressed and localizes to the leading edge of cell protrusions in migrating fibroblasts. Both Coronin-1B and Coronin-1A interaction with Arp2/3 complex may be regulated by phosphorylation. PKC phosphorylates the N-terminus at Ser-2 , and this phosphorylation reduces interactions with Arp2/3 leading to diminshed cell motility.
Sequence: Coronin-1B (Ser-2) synthetic peptide corresponds to amino acids surrounding serine 2 in human coronin-1B. This sequence has high homology to the conserved site in rat and mouse coronin-1B and coronin-1A. However, this sequence has low homology to the conserved region in other coronins.
Specificity: This peptide is specifically recognized by Coronin-1B (Ser-2) phospho-specific antibody (CP2621) in ELISA, and has been shown to block the reactivity of CP2621 in Western blot and is recommended for blocking in immunocytochemistry.
Buffer/Storage:
Blocking Peptide is supplied in 50µl phosphate-buffered saline and 0.05% sodium azide. Store at –20°C. Stable for 1 year.
Coronins are highly-conserved F-actin binding proteins that play important roles in lamellipodial protrusion during various types of cell motility. In yeast, coronins regulate cytoskeletal changes through inhibition of Arp2/3 complex. Human coronins have been classified in three subgroups type I (coronin-1A, -1B, -1C), type II (coronin-2A, -2B), and type III (coronin-7). These coronins have at least one large b-propeller region that mediates protein-protein interactions and type I and II coronins have coiled-coil regions involved in oligiomerization. Coronin-1B is ubiquitously expressed and localizes to the leading edge of cell protrusions in migrating fibroblasts. Both Coronin-1B and Coronin-1A interaction with Arp2/3 complex may be regulated by phosphorylation. PKC phosphorylates the N-terminus at Ser-2 , and this phosphorylation reduces interactions with Arp2/3 leading to diminshed cell motility.
Sequence: Coronin-1B (Ser-2) synthetic peptide corresponds to amino acids surrounding serine 2 in human coronin-1B. This sequence has high homology to the conserved site in rat and mouse coronin-1B and coronin-1A. However, this sequence has low homology to the conserved region in other coronins.
Specificity: This peptide is specifically recognized by Coronin-1B (Ser-2) phospho-specific antibody (CP2621) in ELISA, and has been shown to block the reactivity of CP2621 in Western blot and is recommended for blocking in immunocytochemistry.
Buffer/Storage:
Blocking Peptide is supplied in 50µl phosphate-buffered saline and 0.05% sodium azide. Store at –20°C. Stable for 1 year.
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