DISC1 (a.a. 740-753) Blocking Peptide
€155.00
In stock
SKU
ECM-DX3025
Background:
Disrupted in schizophrenia 1 (DISC1) is a susceptibility factor for a wide range of mental illnesses, including schizophrenia, mood disorders, and autism. DISC1 is expressed in both neuronal progenitor cells and postmitotic neurons in the developing cerebral cortex. DISC1 can interact with both centrosomal proteins and dynein-motor related proteins. In addition, DISC1 interacts with and inhibits the kinase GSK-3β to enable Wnt activation of β-catenin–mediated gene transcription and neuron proliferation. These functions of DISC1 may be regulated by post-translational modification. PKA and CDK5 can phosphorylate Ser-710, and a non-phosphorylatable Ser-710 mutant shows decreased interaction with the centrosomal proteins, BBS1 and BBS4, while a constituitively phosphorylated Ser-710 mutant shows increased interaction with these proteins. During neuronal progenitor cell proliferation, DISC1 phosphorylation at Ser-710 is low and the interaction with GSK-3β is enhanced. By contrast, DISC1 phosphorylation is increased, association with GSK-3β is decreased, and interaction with BBS1 is enhanced during postmitotic neuron migration.
Sequence: DISC1 synthetic peptide containing amino acid residues 740 to 753 from mouse DISC1. This peptide sequence is well conserved in rat DISC1, but has 7 amino acid differences with the conserved site in human DISC1.
Specificity: The peptide is specifically recognized by anti-DISC1 (a.a. 740-753) antibody (DP3021 and DP3041) in ELISA, and has been shown to block the reactivity of DP3021 and DP3041 during Western blot. In addition, the peptide is recommended for use in blocking DP3021 and DP3041 reactivity in immunocytochemistry.
Buffer/Storage:
Blocking Peptide is supplied in 50µl phosphate-buffered saline and 0.05% sodium azide. Store at –20°C. Stable for 1 year.
Disrupted in schizophrenia 1 (DISC1) is a susceptibility factor for a wide range of mental illnesses, including schizophrenia, mood disorders, and autism. DISC1 is expressed in both neuronal progenitor cells and postmitotic neurons in the developing cerebral cortex. DISC1 can interact with both centrosomal proteins and dynein-motor related proteins. In addition, DISC1 interacts with and inhibits the kinase GSK-3β to enable Wnt activation of β-catenin–mediated gene transcription and neuron proliferation. These functions of DISC1 may be regulated by post-translational modification. PKA and CDK5 can phosphorylate Ser-710, and a non-phosphorylatable Ser-710 mutant shows decreased interaction with the centrosomal proteins, BBS1 and BBS4, while a constituitively phosphorylated Ser-710 mutant shows increased interaction with these proteins. During neuronal progenitor cell proliferation, DISC1 phosphorylation at Ser-710 is low and the interaction with GSK-3β is enhanced. By contrast, DISC1 phosphorylation is increased, association with GSK-3β is decreased, and interaction with BBS1 is enhanced during postmitotic neuron migration.
Sequence: DISC1 synthetic peptide containing amino acid residues 740 to 753 from mouse DISC1. This peptide sequence is well conserved in rat DISC1, but has 7 amino acid differences with the conserved site in human DISC1.
Specificity: The peptide is specifically recognized by anti-DISC1 (a.a. 740-753) antibody (DP3021 and DP3041) in ELISA, and has been shown to block the reactivity of DP3021 and DP3041 during Western blot. In addition, the peptide is recommended for use in blocking DP3021 and DP3041 reactivity in immunocytochemistry.
Buffer/Storage:
Blocking Peptide is supplied in 50µl phosphate-buffered saline and 0.05% sodium azide. Store at –20°C. Stable for 1 year.
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