Dok-2 (phospho-Y299) polyclonal, anti-human, mouse, rat
€328.00
In stock
SKU
BS4786
Background:
Dok-1 associates with the Ras GTPase activating protein (Ras GAP) upon tyrosine phosphorylation. Evidence suggests that p62 Dok-1 is a substrate of the constitutive tyrosine kinase activity of p210 Bcr-Abl, a fusion protein caused by the t(9;22) translocation and associated with chronic myelogenous leukemia. Dok-1, as well as the tyrosine kinase substrates IRS-1 and Cas, is a member of a class of “docking” proteins which contain multiple tyrosine residues and putative SH2 binding sites. Dok-1 is suspected to be the substrate phosphorylated in response to stimulation by a number of growth factors, including PDGF, VEGF, insulin and IGF. Dok-2 (also designated p56 Dok) has also been identified as a potential mediator of the effects of p210 Bcr-Abl.
Alternative Name:
Docking protein 2, Downstream of tyrosine kinase 2, p56(dok-2), DOK2
Application Dilution: WB: 1:500~1:1000, IHC: 1:50~1:200
Specificity: p-Dok-2 (Y299) polyclonal antibody detects endogenous levels of Dok-2 protein when phosphorylated at Tyr299.
Immunogen:
Synthetic phosphopeptide derived from human Dok-2 around the phosphorylation site of Tyrosine 299.
MW: ~ 56 kDa
Swis Prot.: O60496
Purification & Purity:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Format:
1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
For research use only, not for use in diagnostic procedure.
Dok-1 associates with the Ras GTPase activating protein (Ras GAP) upon tyrosine phosphorylation. Evidence suggests that p62 Dok-1 is a substrate of the constitutive tyrosine kinase activity of p210 Bcr-Abl, a fusion protein caused by the t(9;22) translocation and associated with chronic myelogenous leukemia. Dok-1, as well as the tyrosine kinase substrates IRS-1 and Cas, is a member of a class of “docking” proteins which contain multiple tyrosine residues and putative SH2 binding sites. Dok-1 is suspected to be the substrate phosphorylated in response to stimulation by a number of growth factors, including PDGF, VEGF, insulin and IGF. Dok-2 (also designated p56 Dok) has also been identified as a potential mediator of the effects of p210 Bcr-Abl.
Alternative Name:
Docking protein 2, Downstream of tyrosine kinase 2, p56(dok-2), DOK2
Application Dilution: WB: 1:500~1:1000, IHC: 1:50~1:200
Specificity: p-Dok-2 (Y299) polyclonal antibody detects endogenous levels of Dok-2 protein when phosphorylated at Tyr299.
Immunogen:
Synthetic phosphopeptide derived from human Dok-2 around the phosphorylation site of Tyrosine 299.
MW: ~ 56 kDa
Swis Prot.: O60496
Purification & Purity:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Format:
1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
For research use only, not for use in diagnostic procedure.
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