EGFR (phospho-Y845) polyclonal, anti-human
€428.00
In stock
SKU
BS6349
Background:
EGFR (Epidermal growth factor receptor, ErbB-1) is a receptor tyrosine kinase (RTK) that is one of four members of the EGFR/ErbB family of receptor tyrosine kinases. EGFR plays a key role in the regulation of essential normal cellular processes and in the pathophysiology of hyperproliferative diseases such as cancer. Activation of the EGFR signaling pathway has been linked with increased cell proliferation, angiogenesis, metastasis and decreased apoptosis. Phosphorylation of EGF receptor (EGFR) at Tyr845 in the kinase domain is implicated in stabilizing the activation loop, maintaining the active state enzyme, and providing a binding surface for substrate proteins. c-Src is involved in phosphorylation of EGFR at Tyr845. The SH2 domain of PLCγ binds at phospho-Tyr992, resulting in activation of PLCγ-mediated downstream signaling.
Alternative Name:
Epidermal growth factor receptor, EC=2.7.10.1, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, ERBB, ERBB1, HER1, EGFR
Application Dilution: WB: 1:500~1:2000
Specificity: p-EGFR (Y845) polyclonal antibody detects endogenous levels of EGFR protein only when phosphorylated at Tyr845 (removal of the signal peptide).
Immunogen:
Synthetic phosphopeptide derived from human EGFR around the phosphorylation site of Tyrosine 845.
MW: ~ 175 kDa
Swis Prot.: P00533
Purification & Purity:
The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Format:
1 mg/ml in Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2.
Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
For research use only, not for use in diagnostic procedure.
EGFR (Epidermal growth factor receptor, ErbB-1) is a receptor tyrosine kinase (RTK) that is one of four members of the EGFR/ErbB family of receptor tyrosine kinases. EGFR plays a key role in the regulation of essential normal cellular processes and in the pathophysiology of hyperproliferative diseases such as cancer. Activation of the EGFR signaling pathway has been linked with increased cell proliferation, angiogenesis, metastasis and decreased apoptosis. Phosphorylation of EGF receptor (EGFR) at Tyr845 in the kinase domain is implicated in stabilizing the activation loop, maintaining the active state enzyme, and providing a binding surface for substrate proteins. c-Src is involved in phosphorylation of EGFR at Tyr845. The SH2 domain of PLCγ binds at phospho-Tyr992, resulting in activation of PLCγ-mediated downstream signaling.
Alternative Name:
Epidermal growth factor receptor, EC=2.7.10.1, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, ERBB, ERBB1, HER1, EGFR
Application Dilution: WB: 1:500~1:2000
Specificity: p-EGFR (Y845) polyclonal antibody detects endogenous levels of EGFR protein only when phosphorylated at Tyr845 (removal of the signal peptide).
Immunogen:
Synthetic phosphopeptide derived from human EGFR around the phosphorylation site of Tyrosine 845.
MW: ~ 175 kDa
Swis Prot.: P00533
Purification & Purity:
The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Format:
1 mg/ml in Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2.
Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
For research use only, not for use in diagnostic procedure.
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