Girdin (Unphosphorylated Ser-1674) Blocking Peptide
€155.00
In stock
SKU
ECM-GX5865
Background:
Girdin, a member of the CCDC88 (Hook related protein) family, is an actin binding protein involved with cell migration and maintaining cytoskeletal organization. Girdin has conserved domains at the N- and C-terminus that bind microtubules and actin, respectively. It enhances PI3-kinase dependent phosphorylation of proteins, most notably Akt. This same activity can contribute to tumor proliferation, invasion, and metastasis in breast, ovarian, prostate, and pancreatic tissues. Girdin is phosphorylated at three separate locations: Ser-1416, Ser-1674, and Tyr-1764. Ser-1416 is the primary Akt phosphorylation site, while Cyclin-dependent kinases interact with Girdin and phosphorylate Ser-1674. Multiple receptor tyrosine kinases can bind girdin and phosphorylate Tyr-1764.
Background References
Simpson, F. (2005) Traffic. 6(6):442
Jiang, P. (2008) Cancer Res. 1;68(5):1310
Lin, C. (2011) Sci Signal. 4(192): ra64
Lin, C. et al. (2014) Mol Biol Cell. 25(22):3654
Bhandari, D. et al. (2015)Proc Natl Acad Sci. 112(35):E4874
Hayano S. et al, (2015) J Mol Cell Cardiol. 88:55
Sequence: Unphosphorylated Girdin (Ser-1674) synthetic peptide corresponding to amino acid residues around serine 1674 in human Girdin. This sequence has high homology to rat and mouse Girdin, and has low homology to similar regions in other Girdin family members.
Specificity: The peptide is an unphosphorylated control peptide for GX5785. The peptide is recommended for use in ELISA and for blocking antibody reactivity in Western blot and immunocytochemistry.
Application dilution:
Blocking: 1:1000
ELISA: 50 ng/well
End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1 hour at room temperature.
Buffer/Storage:
Blocking Peptide is supplied in 50µl phosphate-buffered saline and 0.05% sodium azide. Store at –20°C. Stable for 1 year.
Girdin, a member of the CCDC88 (Hook related protein) family, is an actin binding protein involved with cell migration and maintaining cytoskeletal organization. Girdin has conserved domains at the N- and C-terminus that bind microtubules and actin, respectively. It enhances PI3-kinase dependent phosphorylation of proteins, most notably Akt. This same activity can contribute to tumor proliferation, invasion, and metastasis in breast, ovarian, prostate, and pancreatic tissues. Girdin is phosphorylated at three separate locations: Ser-1416, Ser-1674, and Tyr-1764. Ser-1416 is the primary Akt phosphorylation site, while Cyclin-dependent kinases interact with Girdin and phosphorylate Ser-1674. Multiple receptor tyrosine kinases can bind girdin and phosphorylate Tyr-1764.
Background References
Simpson, F. (2005) Traffic. 6(6):442
Jiang, P. (2008) Cancer Res. 1;68(5):1310
Lin, C. (2011) Sci Signal. 4(192): ra64
Lin, C. et al. (2014) Mol Biol Cell. 25(22):3654
Bhandari, D. et al. (2015)Proc Natl Acad Sci. 112(35):E4874
Hayano S. et al, (2015) J Mol Cell Cardiol. 88:55
Sequence: Unphosphorylated Girdin (Ser-1674) synthetic peptide corresponding to amino acid residues around serine 1674 in human Girdin. This sequence has high homology to rat and mouse Girdin, and has low homology to similar regions in other Girdin family members.
Specificity: The peptide is an unphosphorylated control peptide for GX5785. The peptide is recommended for use in ELISA and for blocking antibody reactivity in Western blot and immunocytochemistry.
Application dilution:
Blocking: 1:1000
ELISA: 50 ng/well
End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1 hour at room temperature.
Buffer/Storage:
Blocking Peptide is supplied in 50µl phosphate-buffered saline and 0.05% sodium azide. Store at –20°C. Stable for 1 year.
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