GRF-1 (phospho-Y1087) polyclonal, anti-human
€328.00
In stock
SKU
BS4814
Background:
GRF-1 (glucocorticoid receptor DNA-binding factor 1), also known as p190RhoGAP or simply p190, is a transcriptional regulator which binds to the promoter region of the glucocorticoid receptor gene and represses its expression. By repressing GR expression, GRF-1 acts to down-regulate Rho signaling, thereby mediating both actin cytoskeletal rearrangements and cell cycle events. Through its GAP domain, GRF-1 is thought to affect cytokinesis by regulating Rho activity; a regulation that is controlled by the ubiquination of the GTP binding region and subsequent degradation of GRF-1. Additionally, GRF-1 plays an important role in oligodendrocyte differentiation, a process that is absent in malignant glioma tumors, implicating GRF-1 as a possible tumor suppressor. GRF-1 expression is regulated by glucocorticoids and the expressed protein exists as two isoforms produced by alternative splicing events.
Alternative Name:
Rho GTPase-activating protein 35, Glucocorticoid receptor DNA-binding factor 1, Glucocorticoid receptor repression factor 1, GRF-1, Rho GAP p190A, p190-A, ARHGAP35, GRF1, GRLF1, KIAA1722
Application Dilution: WB: 1:500~1:1000, IHC: 1:50~1:200
Specificity: p-GRF-1 (Y1087) polyclonal antibody detects endogenous levels of GRF-1 protein only when phosphorylated at Tyr1087.
Immunogen:
Synthetic phosphopeptide derived from human GRF-1 around the phosphorylation site of Tyrosine 1087.
MW: ~ 172 kDa
Swis Prot.: Q9NRY4
Purification & Purity:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Format:
1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
For research use only, not for use in diagnostic procedure.
GRF-1 (glucocorticoid receptor DNA-binding factor 1), also known as p190RhoGAP or simply p190, is a transcriptional regulator which binds to the promoter region of the glucocorticoid receptor gene and represses its expression. By repressing GR expression, GRF-1 acts to down-regulate Rho signaling, thereby mediating both actin cytoskeletal rearrangements and cell cycle events. Through its GAP domain, GRF-1 is thought to affect cytokinesis by regulating Rho activity; a regulation that is controlled by the ubiquination of the GTP binding region and subsequent degradation of GRF-1. Additionally, GRF-1 plays an important role in oligodendrocyte differentiation, a process that is absent in malignant glioma tumors, implicating GRF-1 as a possible tumor suppressor. GRF-1 expression is regulated by glucocorticoids and the expressed protein exists as two isoforms produced by alternative splicing events.
Alternative Name:
Rho GTPase-activating protein 35, Glucocorticoid receptor DNA-binding factor 1, Glucocorticoid receptor repression factor 1, GRF-1, Rho GAP p190A, p190-A, ARHGAP35, GRF1, GRLF1, KIAA1722
Application Dilution: WB: 1:500~1:1000, IHC: 1:50~1:200
Specificity: p-GRF-1 (Y1087) polyclonal antibody detects endogenous levels of GRF-1 protein only when phosphorylated at Tyr1087.
Immunogen:
Synthetic phosphopeptide derived from human GRF-1 around the phosphorylation site of Tyrosine 1087.
MW: ~ 172 kDa
Swis Prot.: Q9NRY4
Purification & Purity:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Format:
1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
For research use only, not for use in diagnostic procedure.
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