Leibovitz's L-15 (Without Phenol Red）

Backgroud: Leibovitz's L-15 medium is composed of phosphate and free base amino acids, replacing the traditional sodium bicarbonate buffer system. At the same time, the use of galactose and sodium pyruvate instead of glucose can prevent the formation of acidic metabolic byproducts, help maintain the stability of the pH of the culture medium, and is suitable for cell culture in non-CO2 balanced environments. Leibovitz's L-15 medium is suitable for the culture of monkey kidney cells and HEp-2, the separation of primary cells (such as embryonic and adult tissues), the culture of various viruses, and the culture of neurons.

Phenol red is used as a pH indicator in culture media to continuously monitor the pH of the culture medium. At low pH values, phenol red makes the culture medium yellow, while at higher pH values, the culture medium turns purple. It turns red at pH 7.2-7.4, which is most suitable for cell culture. However, phenol red also has some disadvantages. Studies have shown that phenol red can simulate the effects of steroid hormones (especially estrogen). Therefore, when using estrogen-sensitive cells (such as breast tissue), it is best to use a culture medium that does not contain phenol red. Phenol red can interfere with detection during flow cytometry analysis. In addition, the presence of phenol red in some serum-free culture medium formulas can interfere with sodium-potassium balance.

Contains: L-Glutamine: 300mg/L, NaHCO3, D-GlucoseWithout, Sodium Pyruvate550mg/L, HEPESWithout, Phenol RedWithout, Balanced Salt SolutionBalanced salt Phosphate, NucleosideWithout, NEAAWithout, D-Galactose900mg/L

Shipping: Room Temperature

Storage: Store at 2-8°C away from light

Shelf Life: 12 months